For salt acetate 8.2g/ml but I desire the end volume to be 500ml so i only include 4.1g in 400ml distilled water
I will certainly prepare 0.1M the acetic mountain from 100% acetic acid (17.4M) V = 0.1M (1000ml) and add it into sodium acetate till i get pH4.5

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On predicting the pH that pure sodium acetate aq. Sol.; see: https://www.moment-g.com/post/Can_we_keep_the_pH_of_3M_Sodium_Acetate_around_7_instead_45-5_while_using_this_in_DNA_extraction_from_whole_blood

if you want to prepare 0.1M Acetic mountain from glacial acetic mountain (=100%), you have actually the density of 1.049 g/mL for the mountain (see link). With the molar fixed of 60.05 g·mol−1 (the molar concentration that glacial acetic mountain is about 0.01747 mol/mL = 17.47M).

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To prepare 1 together of 0.1M solution, you require 0.1 mol glacial acetic acid, i beg your pardon is 6.005g. Through the known density, you need 5.72 mL glacial acetic acid which you need to fill approximately 1L. If you execute not have accessibility to glacial acetic acid, you need a higher volume of the acid equipment (e.g. If you have actually a 80% solution, you require 7.15 ml).
With the given tables native Mushtaq, you just need come mix the best volumes then. Double check the pH of her buffer afterwards!
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if you desire to prepare 0.1M Acetic mountain from glacial acetic mountain (=100%), you have the thickness of 1.049 g/mL because that the acid (see link). V the molar fixed of 60.05 g·mol−1 (the molar concentration that glacial acetic mountain is approximately 0.01747 mol/mL = 17.47M).

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To prepare 1 l of 0.1M solution, you need 0.1 mol glacial acetic acid, i m sorry is 6.005g. V the recognized density, you need 5.72 mL glacial acetic mountain which you need to fill up to 1L. If you execute not have accessibility to glacial acetic acid, you require a higher volume of the acid solution (e.g. If you have a 80% solution, you require 7.15 ml).
With the given tables from Mushtaq, you just need come mix the best volumes then. Double check the pH of your buffer afterwards!
In fact you haven't made clear wither you desire a buffer the pH 4.5, ready from sodium acetatate-acetic acid, then you deserve to use the tables provided to friend by Mushtaq Ahmed. But if you desire that the buffer of 4.5 must be that 0.1M NaAc in the final volume the 500 mL, climate the instance will be different. Below I describe this come you:
First, the 8.2 have to be g/L and also not g/mL. As you have given. However, to have the concentration that NaAc = 0.1M, the the 400 mL you add an initial must it is in 0.125M to become 0.1M in the final solution the 500 mL. For Ac. You have actually only 100 mL room left to have pH that 4.5, because of this M that Ac need to be around 0.2M or slightly higher, then include it progressively with good stirring of food immersing pH electrode in the solution and continue adding it until pH=4.5. The is vital to have actually the Ac in a 100 ml volume vol. Flask. After correct pH has actually been reached, If only few drops of D.water were necessary to complete volume in the volumetric flask, that will certainly not do any far-ranging error. If big space remained, it might be necessary to have actually the pH a little higher 4.51 or 4.52,..... To compensate because that that. The will depend to exactly how accurate carry out you want to be that pH, girlfriend can manage it surely. Give thanks to You and an excellent luck.