Part 1: Orientation of pictures in the MicroscopePart 2: exercise with Depth of field in the MicroscopePart 3: examination of Pond Water & microorganisms

(Adapted from

Parts of the Microscope:



Your microscope has 4 objective lenses: Scanning (4x), short (10x), High (40x), and also Oil Immersion (100x). In this lab, you will certainly not usage the oil immersion lens; that is for viewing microorganisms and also requires technological instructions not covered in this procedure.

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In enhancement to the objective lenses, the ocular lens (eyepiece) has actually a magnification. The complete magnification is identified by multiply the magnification the the ocular and objective lenses.

Magnification Ocular Lens Total Magnification
Scanning 4x 10x 40x
Low Power 10x 10x 100x
High Power 40x 10x 400x
Oil Immersion 100x 10x 1000x

General Procedures:

1. Make sure all backpacks, purses, etc. Room off the benchtop.

2. Lug the microscopic lense by the base and also arm through both hands.

3. Store with the cord wrapped approximately the microscope and the scanning target clicked into place.

Focusing Specimens:

1. Plug her microscope into the power supply and switch top top the illuminator.

2. Always start through the phase as low as feasible and utilizing scanning target (4x). Odds are, you will have the ability to see something on this setup (sometimes it’s just a color). Usage the outlet knob to focus: the image may be little at this magnification, however you won"t have the ability to find it on the higher powers without this an initial step. Move the mechanical stage until your focused image is additionally centered.

3. When you"ve focused using the scanning objective, switch to the low power objective (10x). Usage the coarse knob to refocus and move the mechanical stage to re-center her image. Again, if you haven"t focused on this level, you will certainly not have the ability to move come the following level.

4. Currently switch to the high power objective (40x). In ~ this point, only use the good adjustment knob to emphasis specimens.

5. If the specimen is also light or as well dark, shot adjusting the diaphragm.


1. Store microscope with the scanning target in place and the phase in its shortest position.

2. Plunder cords approximately the microscope.

3. Change slides to original slide tray.


Compound microscope Microscope slide through the letter “e”


Compound microscopic lense Microscope slide with 3 threads


Compound microscope Microscope on slide Coverslip transport pipette Pond water sample


1. Utilizing the deliver pipette, carry a drop of pond water top top a microscopic lense slide. The finest specimens commonly come from the bottom and probably will certainly contain chunks of birds or various other debris the you deserve to see v your nude eye.

2. Ar the coverslip ~ above the slide.

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3. Usage the SCANNING (4x) objective to focus, then move the mechanical stage approximately to scan the slide because that live microorganisms. Girlfriend are searching for tiny swimming beings- they might look eco-friendly or clear and also might be an extremely small. Choose one to emphasis on and also center the in your visual field.


You might wish to usage the ProtoSlo to store your organisms from swimming also quickly!